Описание
Benson: Microbiological
Applications Lab Manual,
Eighth Edition
Companies, 2001
This eighth edition of Microbiological Applications
differs from the previous edition in that it has acquired
four new exercises and dropped three experiments. It
retains essentially the same format throughout, however. In response to requests for more emphasis on laboratory safety, three new features have been incorporated into the text. In addition, several experiments
have been altered to improve simplicity and reliability.
The three exercises that were dropped pertain to flagellar staining, bacterial conjugation, and nitrification in
soil. All of these exercises were either difficult to perform, unreliable, or of minimal pedagogical value.
To provide greater safety awareness in the laboratory, the following three features were added: (1) an
introductory laboratory protocol, (2) many cautionary
boxes dispersed throughout the text, and (3) a new exercise pertaining to aseptic technique.
The three-page laboratory protocol, which follows this preface, replaces the former introduction. It
provides terminology, safety measures, an introduction to aseptic technique, and other rules that apply to
laboratory safety.
To alert students to potential hazards in performing
certain experiments, caution boxes have been incorporated wherever they are needed. Although most of these
cautionary statements existed in previous editions, they
were not emphasized as much as they are in this edition.
Exercise 8 (Aseptic Technique) has been structured to provide further emphasis on culture tube handling. In previous editions it was assumed that students
would learn these important skills as experiments were
performed. With the risk of being redundant, six pages
have been devoted to the proper handling of culture
tubes when making inoculation transfers.
Although most experiments remain unchanged,
there are a few that have been considerably altered.
Exercise 27 (Isolation of Anaerobic Phototrophic
Bacteria), in particular, is completely new. By using
the Winogradsky column for isolating and identifying
the phototrophic sulfur bacteria, it has been possible
to greatly enrich the scope of this experiment. Another
exercise that has been altered somewhat is Exercise
48, which pertains to oxidation and fermentation tests
that are used for identifying unknown bacteria.
The section that has undergone the greatest reorganization is Part 10 (Microbiology of Soil). In the
previous edition it consisted of five exercises. In this
edition it has been expanded to seven exercises. A
more complete presentation of the nitrogen cycle is offered in Exercise 58, and two new exercises (Exercises
61 and 62) are included that pertain to the isolation of
denitrifiers.
In addition to the above changes there has been
considerable upgrading of graphics throughout the
book. Approximately thirty-five illustrations have been
replaced. Several critical color photographs pertaining
to molds and physiological tests were also replaced to
bring about more faithful color representation.
I am greatly indebted to my editors, Jean Fornango
and Jim Smith, who made the necessary contacts for
critical reviews. As a result of their efforts the following
individuals have provided me with excellent suggestions for improvement of this manual: Barbara Collins
at California Lutheran University, Thousand Oaks, CA;
Alfred Brown of Auburn University, Auburn, AL;
Lester A. Scharlin at El Camino College, Torrance, CA;
and Hershell Hanks at Collin County Community
College, Plano, TX.
Preface
Детали
- Год издания
- 2001
- Format